Common Principle of all the rapid diagnostic tests:
Currently available Rapid Diagnostic Tests comprise of a base membrane such as nitrocellulose. A detector reagent (antigen/antibody–indicator complex) specific to the analyte, impregnated at one end of the membrane. A capture reagent is coated on the membrane at the test region.
When the specimen is added to the sample pad, it rapidly flows through the conjugate pad. Analyte, if present in the specimen, binds to the detector reagent. As the specimen passes over the test band to which the capture reagent is coated, the analyte-detector reagent complex is immobilized. A colored band proportional to the amount of analyte present in the sample, develops. The excess unbound detector reagent moves further up the membrane and is immobilized at the control band.
For the past ten years, the number of dengue cases has gradually increased in India. According to the statistics, India in 2017 has seen 11,832 more cases of dengue compared with 2016. As healthcare facilities in many dengue endemic regions lack laboratory support, early dengue diagnosis must rely on either clinical recognition or a bedside diagnostic test.
Dengue fever is a severe, flu-like illness that affects infants, young children and adults, but seldom causes death. It is transmitted by the bite of a mosquito infected with one of the four dengue virus serotypes.
The immune response to this virus includes the production of IgM antibodies by the 5th day of symptoms, which remain in the circulatory system for 30-60 days. IgG antibodies appear by the 14th day of infection and persist for life. A secondary infection often results in high fever and, in many cases, initiates hemorrhagic events and circulatory failure. A secondary infection also induces an IgM antibody response after 20 days of infection and IgG antibodies rise within 1-2 days after the onset of symptoms.
Therefore, patients with secondary infections will have a positive IgG result, usually with a positive IgM result as well.
Thus, the use of a reliable and sensitive rapid serological test that can simultaneously detect the presence of anti-dengue IgG and IgM antibodies is of great clinical utility. The DenguCheck provides an excellent methodology for specifically detecting anti-dengue IgG and IgM antibodies. The presence of high titers of IgG antibodies on the Dengue rapid test does not interfere with the detection of IgM antibodies in the sample. By using a mixture of highly purified dengue proteins, the Dengue rapid test is able to detect all 4 Dengue serotypes.
This Dengue Rapid Test is a new generation immunochromatographic test using recombinant dengue viral antigens of all four serotypes to detect specific antibody response. Serum, plasma or whole blood samples may be used with this
This summer, I had the opportunity to intern at Zephyr Biomedicals, Goa where I received the thorough understanding of the company’s product “DenguCheck – Rapid Diagnostics Test for Dengue” and had to come up with effective measures that, would aid the laboratory personnel minimize erroneous reporting and troubleshoot confidently for confident result reporting.
Author : Mahima Varwandkar (2016 batch)